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Master Thesis

Recrutement de CD2AP par les complexes de réplication du virus de l’hépatite C

Abstract : Hepatitis C virus (HCV) is a small, enveloped, single-stranded positive-sense RNA virus. It infects human hepatocytes and its genome is replicated within cytoplasmic replication complexes. According to preliminary results, CD2AP, a soluble protein composed of three SH3 domains, a proline-rich domain, an actin-binding motif and a coiled-coil domain, is recruited by these complexes. In order to determine the mechanism of CD2AP recruitment, truncated CD2AP mutants were generated and expressed into Huh-7 cells replicating HCV RNA. Confocal microscopy studies revealed that SH3-1 and SH3-2 domains were responsible of CD2AP recruitment. PXPXPR motifs have been reported to specifically interact with CD2AP SH3 domains. One such motif was found in HCV genome, just upsteam of the third domain of NS5A viral protein. A mutated replicon (minimal replication unit of the viral genome) was generated to test the importance of this motif. The endogenous CD2AP protein was not recruited anymore by replication complexes of the mutated replicon, suggesting that CD2AP recruitment is caused by direct interaction with NS5A. Co-immunoprecipitation experiments would be necessary to confirm our hypothesis.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-02370341
Contributor : Agrocampus Ouest <>
Submitted on : Tuesday, November 19, 2019 - 1:50:25 PM
Last modification on : Monday, July 6, 2020 - 3:38:27 PM
Long-term archiving on: : Thursday, February 20, 2020 - 5:51:21 PM

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  • HAL Id : dumas-02370341, version 1

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Esther Martin de Fourchambault. Recrutement de CD2AP par les complexes de réplication du virus de l’hépatite C. Sciences du Vivant [q-bio]. 2019. ⟨dumas-02370341⟩

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