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Master Thesis

Archaea méthanogènes et protozoaires de la cavité buccale : étude préliminaire

Abstract : Objective: The aim of this thesis was to study the relationship between the presence of Trichomonas tenax, Entomoeba gingivalis and Methanobacter oralis in periodontal pockets and the correlation to periodontal disease. Method: Twelve periodontal pocket samples were taken: six from patients with periodontal disease and six from control patients. The samples were examined by optical microscopy without preparation and analyzed in molecular biology. Results: Methanobacter oralis was detected on 4/12 (33%) samples taken, including 3 in patients with periodontal pockets. A protozoan was observed by optical microscopy in 1 specimen where molecular biology did not reveal archaea. It was not possible to differentiate Trichomonas tenax or Entamoeba gingivalis. Discussion and conclusion: The small number of samples did not allow statistically significant conclusions to be drawn. In addition the low number of protozoa observed pushes us to reevaluate our detection techniques.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-02394417
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Submitted on : Wednesday, December 4, 2019 - 5:27:57 PM
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Stéphane Delenne. Archaea méthanogènes et protozoaires de la cavité buccale : étude préliminaire. Chirurgie. 2019. ⟨dumas-02394417⟩

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