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Master Thesis

Étude moléculaire du gène G complet du virus respiratoire syncytial humain (HRSV) groupe A circulant en Normandie : quinze ans d’évolution

Abstract : Human respiratory syncytial virus (HRSV) is one of the leading agents involved in acute respiratory infections in pediatric patients. There is a great diversity in HRSV genotypes between and within subgroups A and B. HRSV is a RNA virus and experiences frequent genetic modifications. The actual challenge is to develop an effective and safe vaccine and antiviral therapy against HRSV infections. Therefore, molecular epidemiology studies are important to obtain information about circulating genotypes and virus evolution. In this study, complete G gene, coding for surface glycoprotein, of HRSV-A was sequenced. 524 respiratory samples collected during 15 winters (2003-2018) in Normandy region in France were included. HRSV-A were detected by molecular biology assays and then amplified into 2 fragments with 2 primer pairs. These 2 fragments were sequenced and assembled using Sequencher software into one sequence (894 to 966 nucleotides). Phylogenetic analysis of 297 complete G gene sequences using MEGA6, with Neighbor Joining method were performed to infer trees. From 2003 to 2010, major genotype circulating was GA2. ON1 strain, a novel genotype with a 72 nucleotide G gene duplication, was first reported in 2010 in Ontario. In the present study, this genotype was detected for the first time in 2011 winter season. The percentage of ON1 strains increased to reach 100% in 2014. A second emergence of ON1 strain in 2014 was identified by Bayesian analysis. This is the first epidemiologic study reporting HRSV-A evolution in France based on complete G gene sequences.
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Master Thesis
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Justine Moriceau. Étude moléculaire du gène G complet du virus respiratoire syncytial humain (HRSV) groupe A circulant en Normandie : quinze ans d’évolution. Sciences pharmaceutiques. 2019. ⟨dumas-02439147⟩



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