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Master Thesis

Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of Influenza A/B virus at emergency department admission: a prospective evaluation during the 2017/2018 influenza season

Abstract : Study objective: To investigate the performance of a rapid RT-PCR assay to detect influenza A/B at emergency department admission. Methods: This single-center prospective study recruited adult patients attending the emergency department for influenza-like illness. Triage nurses performed nasopharyngeal swab samples and ran rapid RT-PCR assays using a dedicated device (cobas® Liat, Roche Diagnostics, Meylan, France) located at triage. The same swab sample was also analyzed in the department of virology using conventional RT-PCR techniques. Patients were included 24 hours-a-day, 7 days-a-week. The primary outcome was the diagnostic accuracy of the rapid RT-PCR assay performed at triage. Results: A total of 187 patients were included over 11 days in January 2018. Median age was 70 years (interquartile range 44 to 84) and 95 (51%) were male. Nine (5%) assays had to be repeated due to failure of the first assay. The sensitivity of the rapid RT-PCR assay performed at triage was 0.98 (95% confidence interval (CI): 0.91 – 1.00) and the specificity was 0.99 (95% CI: 0.94 – 1.00). A total of 92 (49%) assays were performed at night-time or during the weekend. The median time from patient entry to rapid RT-PCR assay results was 46 [interquartile range 36 – 55] minutes. Conclusion: Rapid RT-PCR assay performed by nurses at triage to detect influenza A/B is feasible and highly accurate.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-02543194
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Submitted on : Wednesday, April 15, 2020 - 10:38:05 AM
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Maud Hablot. Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of Influenza A/B virus at emergency department admission: a prospective evaluation during the 2017/2018 influenza season. Human health and pathology. 2020. ⟨dumas-02543194⟩

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