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Master Thesis

Evolution forces involve in aphid populations Aphis gossypii on resistant melon plants

Abstract : Target-gene amplicon sequencing is being more and more exploited high-throughput sequencing application to study diversity and interactions within a population. We studied the dynamic evolution of drift and natural selection forces between two resistant clones of A. gossypii, CUC1 and C6, in two plant genotypes of Cucumis Melo, one containing the Vat gene. Plants were infested with resistant aphid clones at diverse range of departure conditions in twelve of each genotype and sampled at two periods after the infestation. We developed a specific amplicon that contains multiples homozygous SNPs that allow differentiating between clone. This method required to construct and estimate a frequency gradient to verify the power and accuracy of the measured frequencies by the amplicon. The study methods for put in evidence the effect of competition were not either complex or expensive. Overall, the results showed that competition was favorable to CUC1 on susceptible plants and to C6 on resistant plants. We observed that no basic methods are available to study effect of drift. New approaches, either quantifying aphids or the use of models can to disclose the effect of drift in populations.
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  • HAL Id : dumas-02964115, version 1

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Rafael Feriche Linares. Evolution forces involve in aphid populations Aphis gossypii on resistant melon plants. Populations and Evolution [q-bio.PE]. 2018. ⟨dumas-02964115⟩

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