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Master Thesis

Effect of withanolide-containing diet on gut microbial communities and AMP expression in two closely related Lepidoptera species : Heliothis subflexa and Heliothis virescens

Abstract : Every interaction between two organisms is a force driving the adaptation of one to the other and vice versa. This force of adaptation becomes more important as the number of interacting organisms and interaction levels increases. In this study we investigate the impact of withanolides, which are a group of plant secondary metabolites synthesized by Physalis plants, on the microbiota of two insect species in order to shed light on the mechanisms of insect-plant coevolution mediated by endosymbionts. We used the model system composed of the generalist lepidopteran moth Heliothis virescens and its close relative Heliothis subflexa, which is a specialist feeder of Physalis plants. We showed by means of bacterial 16S rRNA gene sequencing that H. subflexa has a more diversified gut microflora than H. virescens. Both gut bacterial communities were largely dominated by the genus Enterococcus. Withanolide supplementation to diet given to larvae had no significant effect on the diversity and richness of gut symbionts. In addition, we assessed the temporal expression levels of two antimicrobial peptides - i-type-lysozyme and attatcin-1 - under withanolide treatments on third instar larvae. We showed that the expression of attacin-1 was not affected by any of the treatments; however, i-type-lysozyme was upregulated after 24 hours.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-02974813
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Submitted on : Monday, November 9, 2020 - 9:42:24 AM
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Dries Amezian. Effect of withanolide-containing diet on gut microbial communities and AMP expression in two closely related Lepidoptera species : Heliothis subflexa and Heliothis virescens. Life Sciences [q-bio]. 2018. ⟨dumas-02974813⟩

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