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Master Thesis

Étude de la réponse T CD4 vis-à-vis du récepteur au N-méthyl-D-aspartate : implications pour l’immunothérapie dans l’encéphalite auto-immune

Abstract : Anti-N-méthyl-D-aspartate receptor (NMDAR) encephalitis is a severe disorder of central nervous system causing neuropsychiatric symptoms. This pathology is mediated by auto-antibodies directed against GluN1 subunit of NMDAR. No information is available on the involvement of CD4 T cells, required for the establishment of a humoral response with high affinity IgG. The objective was to evaluate CD4 T cell response against GluN1 antigen and determine its epitopes. Using bioinformatic analysis we selected ten candidate peptides from GluN1 protein that bind with a strong affinity HLA-DR1 molecule. Then, in vitro experiments has been realized with peripheral blood mononuclear cells of healthy donors stimulated by GluN1 protein or candidate peptides. Our ELISpot results identify two relevant peptides, but without evidences of processing from GluN1 protein. These preliminaries results should be confirm on a higher number of healthy donors. Characterization of CD4 T epitopes of GluN1 would be useful to evaluate the patients’ T cells reponse and development of tolerogenic immunotherapy strategies.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03109094
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Submitted on : Wednesday, January 13, 2021 - 4:04:18 PM
Last modification on : Thursday, February 25, 2021 - 3:24:07 AM
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PETIT-BULTEZ-Gautier.pdf
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  • HAL Id : dumas-03109094, version 1

Citation

Gautier Petit-Bultez. Étude de la réponse T CD4 vis-à-vis du récepteur au N-méthyl-D-aspartate : implications pour l’immunothérapie dans l’encéphalite auto-immune. Sciences pharmaceutiques. 2020. ⟨dumas-03109094⟩

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