Skip to Main content Skip to Navigation
Master Thesis

Étude de la sensibilité au céfépime de souches cliniques du complexe Enterobacter cloacae responsables de bactériémies

Abstract : Cefepime (FEP), 4th generation cephalosporins, is amongst the best choices for the treatment of heavy infections caused by cephaslosporinase producing Enterobacteriaceae (hCase), including Enterobacter cloacae. One of the property of this antibiotic is its low inclination to select 3rd generation cephalosprins mutant-resistant by overproducing the Case (Case) in WT strains. This study will determine the CEC FEP susceptibility and mutant-selection rate. To do so, 86 single clinical strains from bloodcultures in CHU of CAEN between 2017-2018 has been studied. The antibiotic susceptibility (with ESBL detection) has been evaluated by the disk diffusion method and the MIC of the FEP has been determined by broth microdilution method. The bactericidial curves and the FEP mutant-resistant rates (8xMIC) were performed with wild-type strain (WT), ESBL strain and hCase strains. The bactericidials curves were similary between the WT strains and hCase, whatever the FEP concentration tested. On the other hand, the FEP mutants-selection rates were different between the WT strains, hCase and BLSE. The results confirms the good in vitro FEP activity against the CEC WT strains. But the FEP mutants-selection probability seems to be higher in the hCase isolates and ESBL isolates. It highlights the in vivo potential risk in monotherapy and recommends the bitherapy for the heavy infections caused by CEC hCase.
Document type :
Master Thesis
Complete list of metadata

https://dumas.ccsd.cnrs.fr/dumas-03110674
Contributor : Ufr Santé Unicaen <>
Submitted on : Thursday, January 14, 2021 - 5:32:58 PM
Last modification on : Thursday, April 22, 2021 - 3:27:51 AM

File

LANGRIS Hugo.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : dumas-03110674, version 1

Citation

Hugo Langris. Étude de la sensibilité au céfépime de souches cliniques du complexe Enterobacter cloacae responsables de bactériémies. Sciences pharmaceutiques. 2019. ⟨dumas-03110674⟩

Share

Metrics

Record views

12

Files downloads

97