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Master Thesis

Développement des résorptions radiculaires post-traumatiques : étude de l’inflammation dans un modèle animal

Abstract : Introduction. External root resorption is a non bacterian destruction of the dental radicular mineralized tissues. This phenomenon can lead to the tooth loss by lack of therapeutic means. Two requirements are needed to initiate the resorption : the alteration of the protective layer (pre-cementum) and inflammation. Clastic cells are then recruted via the OPG/RANKL/RANK to resorb the radicular tissues. The role played by the pulp inflammation in this process is unknown. Our objective is to propose a murine model of external root resorption to study the role of pulp inflammation and to set it in the contemporary scientific litterature.
Materials and methods. An experimental protocol has been applied to the first maxillary molar of mices. The first modality consisted in applying a pulpal traumatism, the second a periodontal traumatism and the third combined both traumatisms. The animals had been sacrificed at 7, 21 and 42 days. A histological and morphometric analysis has been realized to statistically compare the different groups and times. A bibliographic research enabled to put in perspective this model.
Results. The resorption is a time progressive phenomenon. The group which underwent the periodontal traumatism only shows a significant increase of quantity of resorption through time between d7 and d42. The periodontal traumatism only breeds more resorption than pulpal traumatism only. Indeed, we observed a significant difference between the two groups at d21 and d42. There is no significative difference for the group which combined the two traumatisms.
Discussion. We propose an original animal model of external root resorption that enable the analysis of pulp inflammation’s role. The developped protocol is reproductible and permits the comparison of the samples according to the operating mode and through time. An exploration of specific inflammation markers and the OPG/RANKL/RANK system could enable the understanding of the signaling pathways used by the pulp inflammation and thus determining its role in root resorption. This model meets a topical problematic regarding therapeutics and physiopathology.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03549721
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Submitted on : Monday, January 31, 2022 - 3:33:10 PM
Last modification on : Thursday, April 7, 2022 - 3:06:02 PM
Long-term archiving on: : Sunday, May 1, 2022 - 7:43:49 PM

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Sara Khedhir. Développement des résorptions radiculaires post-traumatiques : étude de l’inflammation dans un modèle animal. Sciences du Vivant [q-bio]. 2021. ⟨dumas-03549721⟩

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