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Master Thesis

Intérêt de l'anticorps Immunoglobulines G Anti Hsp70.1 dans le diagnostic de toxoplasmose oculaire

Abstract : Laboratory diagnosis of ocular toxoplasmosis (OT) needs to be improved mainly in clinical atypical cases. In a retrospective previous study, infected patients could display serum IgG anti-Hsp70.1 antibodies and that these antibodies could be used to improve the diagnosis of OT. The purpose of this multicentre prospective case-control study was to correlate clinical features with IgG anti-Hsp70 antibodies. We have included patients with confirmed (Group A1) or suspected OT (Group A2), and with cataract (Control Group B). The diagnosis was based on the ocular presentation, Goldmann-Witmer's coefficient, immunoblotting and/or PCR. Serum and aqueous humour (AH) were sampled at the time of uveitis. ELISA was used to measure serum anti-Hsp70.1-antibody levels. The OT patients were clinically followed up to six months and laboratory monitored up to three months. Serum IgG anti-Hsp70.1 antibody levels were related to the affected retinal zone (p=0.006) and were correlated significantly to the retinal lesion size (p<0.05). Serum anti-Hsp70.1 and AH IgG anti-Toxoplasma antibodies were respectively positive in 10 and 5 out of 18 cases of Group A2 that were finally classified as true-clinically suspected OT. Anti-Hsp70 may be involved directly or indirectly in the retinal lesions during ocular Toxoplasma infection and related to the affected retinal zone. Anti-Hsp70.1 antibody may confirm biologically suspected OT with an increasing specificity when associated with the presence of AH IgG anti-Toxoplasma antibodies. The value of AH anti-Hsp-70 antibody levels in the laboratory diagnosis of OT will further evaluated.
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Master Thesis
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Submitted on : Tuesday, June 3, 2014 - 3:17:48 PM
Last modification on : Wednesday, July 15, 2020 - 8:58:04 AM
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Antoine Lesoin. Intérêt de l'anticorps Immunoglobulines G Anti Hsp70.1 dans le diagnostic de toxoplasmose oculaire. Médecine humaine et pathologie. 2013. ⟨dumas-00999405⟩

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