Effet d'une restriction alimentaire sur l'aptitude des vaches laitières à développer une réponse pro-inflammatoire

Abstract : In dairy cows, feed restriction is known to decrease milk yield, protein yield and to influence udder metabolism. This study aimed to examine the link between feed restriction and health in dairy cows. Fifty-four Prim’Holstein received a standard feeding for 2 weeks and then were fed a restricted diet for 5 weeks. Milk production and internal temperature were recorded. Blood and milk sample were collected. Mammary epithelial cells (MEC) were purified from milk and used in a bacterial challenge. MEC were incubated with a saline solution, Escherichia Coli or Staphylococcus Aureus. Blood was used in a bacterial challenge too. The concentration of interleukin 8 produced during the challenge was measured. Feed restriction decreased milk yield, fat yield and protein yield. In the blood, feed restriction induced a higher production of IL-8 after bacterial challenge. The bacterial challenge did not work with MEC. Feed restriction also affected the integrity of udder epithelium by opening tight junctions.
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Élisa Arnaud. Effet d'une restriction alimentaire sur l'aptitude des vaches laitières à développer une réponse pro-inflammatoire. Sciences du Vivant [q-bio]. 2018. ⟨dumas-01950742⟩

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