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Master Thesis

Étude par cytométrie en flux des lymphocytes T régulateurs dans les syndromes myélodysplasiques

Abstract : Introduction: the immune system is frequently deregulated in patients with myelodysplastic syndrome (MDS). Many cell types and complex mechanisms are implicated in this deregulation which sustains progression of MDS. Regulatory T cells (Tregs) are known to induce immunosuppression and play a pivotal role in these modifications. The objective of this study was to evaluate and characterize distinct Tregs sub-populations in patients with MDS by flow cytometry. Material and methods: 28 bone marrow samples from patients with MDS classified according the IPSS-R were analysed (Navios, Beckman Coulter) and compared to 22 control samples. First, Tregs were quantified using a manual gating strategy CD3+ CD4+ CD25high CD127low with the Kaluza software. Then, an unsupervised strategy using FlowSOM was used. Results: no significant difference was observed between MDS and control samples according the Tregs quantification. However, a fraction of memory and activated Tregs characterized by the phenotype CD45RA- CD95+ was significantly increased in patients with MDS. Finally, the analysis performed with the FlowSOM algorithm revealed that this CD45RA- CD95+ Tregs population increased in MDS also express CCR4. Conclusion: these results led to the identification of an increased population of Tregs CD45RA- CD95+ in bone marrow of patients with MDS. Interestingly, this population which is reported to have a high immunosuppressive capacity, also express CCR4 probably leading to the specific recruitment of these cells in bone marrow of patients with MDS.
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Master Thesis
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Contributor : Université Paris Descartes - Scd <>
Submitted on : Monday, February 8, 2021 - 4:58:52 PM
Last modification on : Saturday, February 13, 2021 - 3:18:45 AM
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  • HAL Id : dumas-02504125, version 1


Pierre Boncoeur. Étude par cytométrie en flux des lymphocytes T régulateurs dans les syndromes myélodysplasiques. Médecine humaine et pathologie. 2019. ⟨dumas-02504125⟩



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