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Master Thesis

Étude de l’hypersensibilité électromagnétique : effets génotoxiques des ondes radiofréquences

Abstract : In recent years, electromagnetic hypersensitivity (EHS) has become a subject of controversy. This pathology is characterized by a self-diagnosis of patients who attribute their symptoms to millimeter waves (OM) exposures (30 - 300 GHz), but, to date, no causal relationship between exposures and these symptoms has been demonstrated. The purpose of this internship is to determine if OM generate genotoxic stress and compare this response on cells from healthy and electrohypersensitive patients. Commercial skin fibroblasts (HDFA) and samples from electrohypersensible patients, or not, are exposed 14 h at 60 GHz with or without potassium bromate (KBrO3), a known oxidizing agent. Genotoxic stress is studied through the activation of the ATM protein-dependent repair system by immunofluorescence and by RT-qPCR and Western Blot. We observed that ATM activation was impaired for some samples when exposed to OM. In addition, the expression of genes involved in DNA repair was not modified but the level of phosphorylation of certain proteins (XRCC1) increased after exposing the HDFA. This study, although incomplete, opens a trail on the mechanism involved in EHS.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-02649427
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Submitted on : Friday, May 29, 2020 - 12:00:49 PM
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2019_Mahé_Coline_BMC.pdf
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Coline Mahé. Étude de l’hypersensibilité électromagnétique : effets génotoxiques des ondes radiofréquences. Sciences du Vivant [q-bio]. 2019. ⟨dumas-02649427⟩

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