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Master Thesis

Étude de la stabilité microbiologique de trois collyres renforcés en antibiotiques (Amikacine, Ceftazidime et Vancomycine)

Abstract : Fortified antibiotic ophthalmic solutions are emergency treatment for bacterial keratitis. At Bordeaux University Hospital, they were produced weekly because of their short stability at fridge. In order to optimize production, this study evaluates the sterility of 5% solutions of amikacin, ceftazidime and vancomycin stored at -20°C for 3 months and compare their microbiological stability in 2 different containers: High-Density-PolyEthylene (HDPE) bottles and Novelia® bottles. Solutions of 5% amikacin and 5% ceftazidime were diluted in 0,9% sodium chloride and 5% vancomycin in 5% dextrose. Over a 90-day period, physico-chemical characteristics were monitored and in vitro sterility was assayed by the membrane filtration method according to the European Pharmacopoeia (2.6.1) for each solution. The sterility and physical properties were also studied after 4, 8 and 14 days of simulated use. The results of this study demonstrated that 5% amikacin, ceftazidime and vancomycin are physically and microbiologically stable at -20°C for 90 days. After delivery, in HDPE bottles, they must be stored at 2 - 8°C and used within 8 days whereas, in Novelia® bottles, 5% amikacin and vancomycin could be used until 14 days after opening. However, 5% ceftazidime in Novelia® bottles must be used within 8 days after delivery because of the short physico-chemical stability.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03019637
Contributor : Frédérique Gambade <>
Submitted on : Monday, November 23, 2020 - 2:33:05 PM
Last modification on : Thursday, January 21, 2021 - 3:10:31 AM
Long-term archiving on: : Wednesday, February 24, 2021 - 7:22:35 PM

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Lucile Pinon. Étude de la stabilité microbiologique de trois collyres renforcés en antibiotiques (Amikacine, Ceftazidime et Vancomycine). Sciences du Vivant [q-bio]. 2020. ⟨dumas-03019637⟩

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