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Master Thesis

Prévalence et caractérisation phénotypique et génotypique des entérobactéries productrices de béta-lactamase à spectre élargi (E-BLSE) dans les élevages de rente de Mayotte et Madagascar

Abstract : Introduction: the presence of Extended-spectrum β-lactamase producing Enterobacteriaceae (E-ESBL) in livestock farming has been widely reported in several countries. E-ESBL are currently considered as one of the main threats for the treatment of infections in both humans and animals. However, data for distant territories such as Indian Ocean are very scarce. In this study, we aimed to assess the animal E-ESBL prevalence and characterize the strains involved in Madagascar and Mayotte islands. Methods: 133 livestocks farms were sampled using boot swabs. The main livestock sectors were targeted [Madagascar : cattle (n=31) poultry (n=30) pigs (n=30) ; Mayotte : cattle (n=19) poultry (n=23) ]. The microbiological investigations consisted in a phenotypical characterization of the ESBL strains (e.g co-resistances) and a molecular identification of the involved enzyme. Results: high prevalences were observed in all the production sectors [Madagascar : cattle (64,5%) poultry (70%) pigs (83,9%) ; Mayotte : cattle (68,4%) poultry (73,9%) ]. CTX-M15 subtype was the most common in the cattle and pigs farms whereas CTX-M1 were more associated with poultry holdings. High level of co-resistances were highlighted, but no MCR-1 resistance. Discussion: this study was the first to cipher the E-ESBL resistance in these two islands. The high prevalences found reinforce the possibility of an animal reservoir with antimicrobial resistance, and the molecular characterization provides interesting discussion tracks to its origin.
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https://dumas.ccsd.cnrs.fr/dumas-02419081
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Submitted on : Thursday, December 19, 2019 - 12:00:08 PM
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Alexandre Leclaire. Prévalence et caractérisation phénotypique et génotypique des entérobactéries productrices de béta-lactamase à spectre élargi (E-BLSE) dans les élevages de rente de Mayotte et Madagascar. Sciences du Vivant [q-bio]. 2019. ⟨dumas-02419081⟩

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